The QIAsymphony DNA Investigator Kit enables automated purification of genomic DNA from 1–96 samples, such as swabs, filters, casework or crime-scene samples, and blood on the QIAsymphony SP. Purification is fast and efficient, and purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis, with high signal-to-noise ratios.
The QIAsymphony DNA Investigator Kit enables highly efficient purification of DNA, even from very small amounts of sample material. The combination of sample input volumes of up to 1000 µl with elution volumes as low as 30 µl enables highly sensitive detection of DNA from a wide range of sample types, including diffuse stains.
The purification procedure is designed to ensure safe and reproducible handling of precious or potentially infectious samples, and comprises 4 steps: lyse, bind, wash and elute. The user can choose different elution volumes between 30 μl to 400 μl of water or modified TE Buffer (Buffer ATE), depending on the protocol.
Since the type of samples that can be processed using the QIAsymphony DNA Investigator Kit can vary greatly, there is also a variety of different pretreatments, optimized for specific sample types. Samples are lysed under denaturing conditions in the presence of proteinase K and Buffer ATL in total volumes of either 200 μl, 500 μl or 1000 μl.
There are two different kinds of DNA purification protocols, which can be used in conjunction with the pretreatment protocols. Casework protocols purify genomic and mitochondrial DNA from 200 μl, 500 μl or 1000 μl of lysate obtained from the sample lysis procedures detailed in the corresponding pretreatment protocols. DNA can be eluted in 30–200 μl of either water or modified TE Buffer (Buffer ATE). Reference protocols purify DNA from database samples, such as buccal swabs or dried blood. DNA can be eluted in 100–400 μl of Buffer ATE. The purified DNA is ready to use in downstream applications.
The high-quality DNA obtained using the QIAsymphony DNA Investigator Kit is suitable for direct use in forensic, environmental, biodefense, biosecurity, and other applied-testing applications, such as:
- Genotyping, including fingerprinting and paternity analysis
- DNA purification from trace samples (e.g., crime-scene)
- DNA extraction from filters used in environmental testing
- Routine analysis of reference samples
|STR analysis, (real-time) PCR
|< 3 µg
|Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein
|Genomic DNA, mitochondrial DNA
|Reference samples: 100-400 µl; Casework samples: 30- 200 µl
|Wide range of forensic and human-identity sample materials
|Magnetic particle technology
|Reference samples: 200 or 500 µl lysate; Casework samples: 200, 500 or 1000 µl lysate
FEATURES & BENEFITS
- Purification from forensic and human identity samples
- More efficient yields from trace casework samples
- Higher signal-to-noise ratios
- Novel, prefilled reagent cartridges for ease of use
- High reproducibility through standardized processing
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|Units of measurement
|QIAGEN® QIAsymphony DNA Investigator Kit
|192 preps of 200 µl each