QIAsymphony SP System enables sample preparation of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials. The QIAsymphony AS extends the capabilities of the QIAsymphony SP by integrating automated PCR assay setup, which, in combination with the Rotor-Gene Q and QIAGEN real-time and end-point PCR kits, enables you to optimize your PCR workflow and maximize your efficiency.
Procedure
The QIAsymphony SP is ready-to-run and requires only minimal installation. Laboratories already operating the SP model can easily upgrade their system onsite with a QIAsymphony AS module integration. The AS model directly interfaces with the SP model. To reduce manual handling steps and minimize the risk of sample contamination, samples processed on the QIAsymphony SP can be transferred automatically to the QIAsymphony AS (integrated operation). For extra flexibility, each of the QIAsymphony SP and AS can also be operated independently. Samples that have been processed earlier in the day, or that have been processed on a different QIAsymphony SP, may be manually transferred to the QIAsymphony AS.
Sample preparation and assay setup runs are defined using the touchscreen. Simply load drawers with the required samples, reagents, and consumables. When each drawer is closed, a detailed inventory scan is performed. This ensures that the worktable is correctly set up for the selected protocols. After sample preparation, either remove purified nucleic acids from the eluate drawer or automatically transfer eluates to the QIAsymphony AS via the transfer module for assay setup. After assay setup, assays can be transferred directly to a real-time PCR cycler for analysis.
The sample input drawer accommodates a wide range of sample input formats to suit varying workflows and sample preparation requirements. Bar code-labeled primary and secondary sample tubes (diameter 10–16.9 mm), microplates, and blocks are supported. Continuous loading in batches of up to 24 samples plus internal controls, and the option to assign different protocols to each batch, delivers increased efficiency and walkaway time. The QIAsymphony AS enables setup of single or multiple PCRs per run or sample, splitting of eluates for panel testing, and active cooling of eluates, reagents, and PCRs. A choice of output adapters enables use of different real-time PCR cyclers (e.g., Rotor-Gene Q, 96-well cyclers, 32-capillary cyclers). To use the Rotor-Gene Q for subsequent detection, simply set up your PCRs in Strip Tubes or Rotor-Discs.
QIAsymphony Kits are safe and exceptionally easy to use. Ready-to-run, bar code-labeled reagent cartridges are prefilled with all reagents required for the purification procedure, including accessory enzymes. Worktable setup is rapid and saves you valuable time. Simply place up to 2 reagent cartridges in the QIAsymphony drawer. The reagent cartridges can be from the same or different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent cartridges are automatically opened by the instrument, and reagent volumes only for the selected number of samples are used, giving you complete cost control.
Principle
Applications
The QIAsymphony SP/AS instruments are suitable for laboratories performing applications, such as:
- Molecular diagnostics research and biomedical research
- Human identity testing
- Veterinary applications
- Biosecurity research
- Life science applications, such as genomics research
Software
QIAsymphony operating software guides the user step-by-step through setting up a sample preparation or assay setup run, with options to select and adapt standardized protocols. Required volumes of buffers, reagents, master mix, and controls are automatically calculated, and the user is informed of the number and type of consumables to be loaded. Transfer of sample loading information from the QIAsymphony SP to the QIAsymphony AS is convenient and reduces the number of steps required for assay definition.
Both of the QIAsymphony instruments provide standardized communication interfaces. Work lists and rack files may be imported to the instruments, enabling automatic run definition before samples are received in the laboratory. After assay setup, sample lists can be exported to selected real-time PCR cyclers. This bi-directional data exchange allows integration of QIAsymphony SP/AS instruments into laboratory information management systems (LIMS).
Specifications
Features | Specifications |
Power | 100–240 V AC, 50–60 Hz, 1400 VA, mains supply voltage are not to exceed 10% of nominal supply voltages |
Input volume | Minimum input volume: 200 µl (application dependent) |
Operating temperature | 15–32°C |
Pollution level | 2 |
Place of operation | For indoor use only |
Elution volume | 30–500 µl (application dependent) |
Kits or Application Packs designed for this instrument | QIAsymphony SP — QIAsymphony DSP Virus/Pathogen Kits and other QIAsymphony Kits |
Overvoltage category | II |
Humidity | Relative humidity of 15–75% (noncondensing); maximum 75% relative humidity for temperatures up to 31°C, decreasing linearly to 50% humidity at 32°C |
Instrument dimensions | QIAsymphony SP, 128 x 103 x 73 cm; QIAsymphony SP/AS (integrated operation), 185 x 103 x 73 cm |
Processing | Automated |
Protocols/main application on this instrument | Purification of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials |
Storage temperature | 5–40°C in manufacturer’s package |
Technology | QIAGEN magnetic-particles chemistry |
Transportation temperature | –25 to 70°C in manufacturer’s package |
Weight | QIAsymphony SP, 175 kg; QIAsymphony SP/AS (integrated operation), 265 kg |
Altitude | Up to 2000 m (6500 ft) |
QIAsymphony SP Parts Also Available
Rod Covers
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Sample Prep Cartridges
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Filter Tips
200 µl
Filter Tips
1500 µl
FEATURES & BENEFITS
- Innovative, easy-to-use modular system with built-in touchscreen.
- Purification of DNA and RNAÂ from a wide range of samples
- Automatic transfer of eluates to the QIAsymphony AS for assay setup
- Continuous sample loading, with bar code reading for sample tracking
- Import of sample lists and export of sample sheets
PRODUCT DETAILS
You may place order by giving us a call
Catalog Number | Description |
---|---|
QG9001297 | QIAGEN® QIAsymphony SP System |
QG997002 | QIAGEN® QIAsymphony SP Prep Cartridges |
QG997004 | QIAGEN® QIAsymphony SP Rod Covers |
QG990332 | QIAGEN® QIAsymphony SP Filter Tips: 200 µl |
QG997024 | QIAGEN® QIAsymphony SP Filter Tips: 1500 µl |