800.676.5565 | [email protected]
Accutest® Custom Pre-Plated Gastrointestinal RT-PCR Panels
Accutest Custom Pre-Plated Gastrointestinal RT-PCR Panels allow research laboratories to select bacterial, viral and parasitic targets and create a panel around their specific molecular research workflow.
Primers and probes are pre-spotted in each well, and MasterMix is included in the current custom-panel configuration. Panels are available in 96-well and 384-well formats with clear or white plate options.
The currently published Accutest GI assay menu includes the targets shown below. Additional assays may be available upon request.
For Research Use Only. Not for use in diagnostic procedures.
17 Published GI Targets
Build from Jant's currently published bacterial, viral and parasitic gastrointestinal assay menu, with additional assays available upon request.
96- & 384-Well Formats
Custom pre-plated panels are available in 96-well and 384-well formats with clear or white plate options.
MasterMix & Internal Control
MasterMix is included, and an internal control is included in every well. RNase P is typically used, with B. atrophaeus available if desired.
Gastrointestinal Target Menu
Use the currently published Accutest GI targets as a starting point for a custom pre-plated RT-PCR configuration. Contact Jant Pharmacal if your research requires additional targets.
Bacterial Targets
- Campylobacter
- Clostridium difficile
- Enteroinvasive Escherichia coli (EIEC)
- Salmonella
- Shiga-like Toxin E. coli stx1
- Shiga-like Toxin E. coli stx2
- Shigella
- Yersinia enterocolitica
Viral Targets
- Astrovirus
- Human rotavirus (HRTV)
- Human sapovirus (HSaV)
- Norovirus G1
- Norovirus G2
- Pan-Adenovirus
Parasitic Targets
- Cryptosporidium
- Entamoeba histolytica
- Giardia lamblia
Custom configuration: Compatible targets from different application categories may be combined on the same custom plate when the chemistry and cycling parameters are compatible. Additional assays may also be available upon request.
Panel Configuration & Ordering Information
Custom panels are produced according to the selected targets, instrument configuration and reaction format. Contact Jant Pharmacal for pricing and final configuration review.
| Specification | Custom Pre-Plated Configuration |
|---|---|
| Plate Formats | 96-well and 384-well |
| Plate Options | Clear or white |
| Primers & Probes | Pre-spotted in each well |
| MasterMix | Included |
| Internal Control | Included in every well; RNase P is typically used, with B. atrophaeus available if desired |
| Positive Control | Available separately at 100,000 copies/µL or 1,000 copies/µL |
| Negative Control | Not included; nuclease-free water is recommended |
| Sample Input | Extracted eluate |
| 96-Well Reaction Volume | Typically 20 µL; certain configurations may support 10 µL reactions |
| 384-Well Reaction Volume | 10 µL |
| Cycling | Standard and fast cycling protocols supported |
| Minimum Order | 50 reactions for 20 µL configurations; 100 reactions for 10 µL configurations |
| Typical Production Time | Approximately 1 week |
| Shipping | Dry ice |
| Storage | –15°C to –25°C in a constant-temperature, non-frost-free freezer |
| Shelf Life | 1 year from manufacture |
Instrument & Fluorescence Compatibility
Confirmed compatible platforms include Bio-Rad, qTOWER and QuantStudio real-time PCR systems. Contact Jant Pharmacal to review compatibility with other RT-PCR instruments.
Real-Time PCR Platforms
Bio-Rad, qTOWER and QuantStudio platforms are confirmed compatible. Compatibility with other systems can be reviewed based on the instrument model and configuration.
Fluorescence Channels
Assays are assigned to fluorophores including FAM, TxRed/ROX, Cy5 and VIC/HEX/SUN according to the selected custom panel configuration.
Build the Complete RT-PCR Workflow
Compare Jant's predefined Gastrointestinal panel or review real-time PCR instrumentation and nucleic acid extraction products for your molecular research workflow.
Standard Gastrointestinal Panel
Compare your custom target requirements with Jant's predefined 17-target Standard Gastrointestinal RT-PCR Panel.
View Standard GI PanelRT-PCR Analyzers
Review real-time PCR instrumentation for new workflows or expanded laboratory capacity.
View RT-PCR AnalyzersNucleic Acid Extraction
Explore manual and automated extraction products for molecular research workflows.
View Extraction SolutionsDocumentation and Resources
Review general instructions for Accutest® Custom Pre-Plated RT-PCR Panels, including storage, reaction setup and cycling information.
Custom Pre-Plated RT-PCR Panels — General Instructions
General storage, reaction setup and PCR cycling information
Frequently Asked Questions
Can I select the gastrointestinal targets included on my custom panel?
Yes. Custom pre-plated panels are configured around the laboratory's selected targets and instrument requirements. The currently published GI menu can be used as a starting point, and additional assays may be available upon request.
Which GI targets are currently published by Jant?
Jant's current GI menu includes 17 bacterial, viral and parasitic targets. The complete published menu is listed above.
How is this different from the Standard Gastrointestinal RT-PCR Panel?
The Standard Gastrointestinal panel uses a predefined 17-target menu. The custom offering allows the laboratory to select targets and develop a configuration around its research and instrument requirements.
Can GI targets be combined with targets from other applications?
Yes. Compatible assays from different application categories may be combined on the same custom plate when the chemistry and cycling parameters are compatible.
Which plate formats are available?
Custom panels are available in both 96-well and 384-well formats with clear or white plate options.
Are the primers and probes already on the plate?
Yes. Primers and probes are pre-spotted in the wells of the custom plate.
Is MasterMix included?
Yes. MasterMix is included with the current custom pre-plated panel configuration.
Are controls included?
An internal control is included in every well. RNase P is typically used, with B. atrophaeus available if desired. Positive controls are available separately, while nuclease-free water is recommended for the negative control.
Which real-time PCR instruments are compatible?
Bio-Rad, qTOWER and QuantStudio systems are confirmed compatible. Contact Jant Pharmacal to review compatibility with other RT-PCR instruments.
What reaction volumes are used?
384-well configurations typically use a 10 µL reaction volume. 96-well configurations typically use 20 µL reactions, although certain 96-well configurations may support 10 µL reactions.
How are custom panels shipped and stored?
Custom pre-plated panels are shipped on dry ice and should be stored at –15°C to –25°C in a constant-temperature, non-frost-free freezer.
What is the minimum order quantity?
The minimum order is 50 reactions when using a 20 µL reaction volume and 100 reactions when using a 10 µL reaction volume.
How long does production typically take?
Typical production time is approximately one week.
Are these panels intended for diagnostic use?
No. These products are offered for Research Use Only and are not for use in diagnostic procedures.
Build Your Custom Gastrointestinal RT-PCR Panel
Call 800.676.5565 or use the form below. Include your desired targets, instrument model, plate format and expected reaction volume when available.