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Quality control (QC) in Next-Generation Sequencing (NGS) is paramount for ensuring the accuracy and reliability of genomic data. Biological and technical factors have the potential to impact both the quality and read-depth of sequencing data. The most common factors include poor quality of starting material, improper library preparation, and technical sequencing errors. Rigorous QC not only ensures the reliability of genomic data but also facilitates downstream analysis and interpretation, ultimately advancing research and clinical applications in genomics.
Quality control (QC) in Next-Generation Sequencing (NGS) is paramount for ensuring the accuracy and reliability of genomic data. Biological and technical factors have the potential to impact both the quality and read-depth of sequencing data. The most common factors include poor quality of starting material, improper library preparation, and technical sequencing errors. Rigorous QC not only ensures the reliability of genomic data but also facilitates downstream analysis and interpretation, ultimately advancing research and clinical applications in genomics.