QIAamp Viral RNA Mini Kit simplifies viral RNA purification from cell-free body fluids with fast spin-column, vacuum and plate centrifugation procedures. Viral RNA binds specifically to the QIAamp silica membrane, and pure viral RNA is eluted in either water or a buffer provided with the kit. Purification can be fully automated on the QIAcube Connect MDx using the QIAamp Viral RNA Mini Kit or dedicated QIAamp Viral RNA Mini QIAcube Kit.
QIAamp Viral RNA Mini standard protocol can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson. The TRACKMAN Connected system guides researchers through QIAamp Viral RNA protocol while automatically adjusting the Bluetooth-enabled PIPETMAN M Connected pipette settings. Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report.
Performance
The high-quality viral RNA isolated using the QIAamp Viral RNA Mini Kit performs well in a wide range of downstream applications, including viral genotyping, viral epidemiology and infectious disease research.
The QIAamp 96 Viral RNA Kit has been successfully used to recover RNA from viruses, including SARS-CoV-2 virus. Performance of the QIAamp 96 Viral RNA Kit is comparable to that achieved with the QIAamp Viral RNA Mini Kit. RNA isolation is proven from commonly used saliva collection and stabilization methods and no cross-contamination was detected
Procedure
Optimized buffers and enzymes lyse samples, stabilize nucleic acids and enhance selective RNA adsorption to the QIAamp membrane. To guarantee RNA integrity, samples are lysed under highly denaturing conditions to inactivate RNases. Alcohol is added and lysates loaded onto the QIAamp spin column or 96-well plate. Wash buffers are used to remove impurities and pure, ready-to-use RNA is then eluted in water or low-salt buffer.
Principle
QIAamp RNA technology yields viral RNA from cell-free body fluids ready to use in RT-PCR and blotting procedures. QIAamp sample preparation technology is fully licensed.
No phenol–chloroform extraction is required. Viral RNA binds specifically to the QIAamp silica membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure viral RNA to be eluted in either water or a buffer provided with the kit.
Applications
QIAamp Viral RNA Kits simplify viral RNA purification from cell-free body fluids, offering fast spin-column, vacuum or plate centrifugation procedures, or automation on QIAcube. Sample types include:
- Plasma and serum
- CSF
- Urine
- Other cell-free body fluids
- Cell-culture supernatants
- Swabs
Comparison of QIAamp Viral RNA Kits
| Features | QIAamp Viral RNA Mini Kit | QIAamp 96 Viral RNA Kit |
|---|---|---|
| Applications | PCR, qPCR, real-time PCR | PCR, qPCR, real-time PCR |
| Elution volume | 50 µl | 80 µl |
| Format | Spin columns | 96-well plate |
| Main sample type | Liquid media | Liquid media |
| Processing | Manual (centrifugation or vacuum) | Manual (centrifugation or vacuum) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Viral RNA | Viral RNA |
| Sample amount | 140 µl | 140 µl solution from a swab sample |
| Technology | Silica technology | Silica technology |
| Time per run or per prep | 20–40 minutes |  |
| Yield | >90% recovery | Â |
FEATURES & BENEFITS
- Rapid isolation of high-quality, ready-to-use RNA
- No organic extraction or alcohol precipitation
- Complete removal of contaminants and inhibitors
- RNA isolation from viruses, such as SARS-CoV-2, up to 96-well format
- Automated purification from low to ultra-high throughput format
PRODUCT DETAILS
You may place order by giving us a call
| Catalog Number | Description | Units of measurement |
|---|---|---|
| QG52906 | QIAGEN® QIAamp Viral RNA Mini Kit | 250 RNA preps |
