Product Overview
The QIAamp® Viral RNA Mini Kit (250) uses silica-membrane spin-column technology to purify viral RNA from plasma, serum, other cell-free body fluids and cell-culture supernatants. Each kit provides components for 250 viral RNA preparations.
Samples are lysed under highly denaturing conditions to inactivate RNases. Viral RNA binds to the QIAamp silica membrane while contaminants pass through. After two wash steps, purified RNA is eluted in RNase-free water or Buffer AVE and is ready for downstream molecular biology applications.
The workflow can be performed manually using centrifugation or vacuum processing and can also be automated on the QIAcube® Connect.
For molecular biology applications. Not intended for the diagnosis, prevention or treatment of disease.
250 RNA Preps
Includes 250 QIAamp Mini Spin Columns, collection tubes, carrier RNA and RNase-free buffers.
20–40 Minute Workflow
Rapid silica-membrane purification with a standard 140 µL sample input.
Flexible Processing
Process samples by centrifugation, vacuum or automated purification on QIAcube Connect.
Key Features
- Rapid purification of high-quality, ready-to-use viral RNA
- Silica-membrane spin-column technology
- No phenol-chloroform extraction or alcohol precipitation
- Two wash steps help remove contaminants and PCR inhibitors
- Standard protocol uses a 140 µL sample input
- 50 µL elution volume
- Manual centrifugation or vacuum processing
- Automatable on the QIAcube® Connect
- Purified RNA is suitable for RT-PCR and other downstream molecular biology applications
Product Specifications
| Jant Catalog Number | QG52906 |
|---|---|
| QIAGEN Catalog Number | 52906 |
| Product Name | QIAamp® Viral RNA Mini Kit (250) |
| Purification Target | Viral RNA |
| Technology | Silica-membrane technology |
| Format | QIAamp Mini Spin Columns |
| Standard Sample Input | 140 µL |
| Sample Types | Plasma, serum, other cell-free body fluids and cell-culture supernatants |
| Elution Volume | 50 µL |
| Processing | Manual centrifugation or vacuum; automatable on QIAcube Connect |
| Typical Workflow Time | Approximately 20–40 minutes |
| Downstream Applications | RT-PCR, PCR, qPCR, real-time PCR and blotting procedures |
| Storage | Store spin columns dry at 15–25°C and avoid higher temperatures. Store solutions at room temperature unless otherwise stated. |
| Intended Use | Molecular biology applications; not intended for diagnosis, prevention or treatment of disease |
| Package Size | 250 RNA preps |
QIAamp Viral RNA Mini Kit vs. QIAamp 96 Viral RNA Kit
Both kits use silica-membrane technology to purify viral RNA, but their formats and processing workflows are designed for different throughput requirements.
| Feature | QIAamp Viral RNA Mini Kit | QIAamp 96 Viral RNA Kit |
|---|---|---|
| Format | Individual spin columns | 96-well plate |
| Sample Input | 140 µL liquid sample | 140 µL solution from a swab sample |
| Elution Volume | 50 µL | 80 µL |
| Processing | Centrifugation, vacuum or QIAcube Connect automation | Plate centrifugation or compatible high-throughput automation |
| Purification Target | Viral RNA | Viral RNA |
| Technology | Silica-membrane technology | Silica-membrane technology |
| Downstream Applications | PCR, qPCR and real-time PCR | PCR, qPCR and real-time PCR |
This product page and the ordering information below are specifically for the QIAamp Viral RNA Mini Kit (250). The QIAamp 96 Viral RNA Kit is a separate product.
Kit Contents
- 250 QIAamp Mini Spin Columns
- 750 Collection Tubes (2 mL)
- 155 mL Buffer AVL
- 98 mL Buffer AW1 concentrate
- 66 mL Buffer AW2 concentrate
- 20 mL Buffer AVE
- 1550 µg Carrier RNA (poly A)
- Components for 250 viral RNA preparations
How Viral RNA Purification Works
The QIAamp Viral RNA Mini procedure combines selective viral RNA binding on a silica membrane with microspin or vacuum processing.
- Lyse: The sample is lysed under highly denaturing conditions to inactivate RNases and help preserve viral RNA.
- Bind: Buffering conditions are adjusted so viral RNA binds to the QIAamp silica membrane.
- Wash: Two wash steps remove contaminants and substances that may inhibit downstream enzymatic reactions.
- Elute: Purified viral RNA is eluted in RNase-free water or Buffer AVE for downstream use.
The same lyse-bind-wash-elute workflow can be performed manually or automated on QIAcube Connect.
Sample Types and Applications
Compatible Sample Types
- Plasma
- Serum
- Other cell-free body fluids
- Cell-culture supernatants
Downstream Applications
- RT-PCR
- PCR and qPCR
- Real-time PCR
- Blotting procedures
- Viral genotyping and infectious disease research
Refer to the current QIAamp Viral RNA Mini Handbook for complete sample-preparation requirements, protocols and limitations.
Related Real-Time PCR Solutions
Purified viral RNA from the QIAamp® Viral RNA Mini Kit can be used in compatible downstream RT-PCR and real-time PCR workflows. Explore Jant Pharmacal’s real-time PCR portfolio, including standard multiplex panels, lyophilized panels, custom pre-plated panels, RT-PCR analyzers and PGx testing options.
Explore Real-Time PCR SolutionsReview the requirements of the selected assay or panel to confirm sample, extraction and workflow compatibility.
Important Use and Safety Information
- For molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of disease.
- Follow appropriate laboratory procedures when handling chemicals and potentially infectious samples.
- Buffers AVL and AW1 contain guanidine salts. Do not add bleach or acidic solutions directly to sample-preparation waste.
- Review the current handbook and applicable safety data sheets before use.
Documentation and Resources
Review the current manufacturer handbook before using the kit.
QIAamp Viral RNA Mini Handbook View Product Information on QIAGENFrequently Asked Questions
The kit purifies viral RNA from plasma, serum, other cell-free body fluids and cell-culture supernatants for downstream molecular biology applications.
The QIAamp Viral RNA Mini Kit (250) includes components for 250 viral RNA preparations.
The standard protocol uses a 140 µL sample input. Consult the manufacturer handbook before processing a different volume because reagent quantities and loading steps may need to be adjusted.
The standard elution volume is 50 µL. Purified RNA may be eluted in RNase-free water or Buffer AVE according to the applicable protocol.
Yes. In addition to manual centrifugation or vacuum processing, the QIAamp Viral RNA Mini Kit can be automated on the QIAcube Connect. Refer to the applicable QIAcube protocol and accessory requirements.
The QIAamp Viral RNA Mini workflow typically takes approximately 20–40 minutes, depending on the processing method and number of samples.
Purified viral RNA is suitable for RT-PCR, PCR, qPCR, real-time PCR, blotting procedures and other compatible molecular biology applications.
Store QIAamp Mini Spin Columns dry at room temperature, 15–25°C, and avoid higher temperatures. Store kit solutions at room temperature unless otherwise stated in the handbook.
No. The kit is intended for molecular biology applications and is not intended for the diagnosis, prevention or treatment of disease.
Ordering Information
To place an order, call Jant Pharmacal at 800.676.5565
| Catalog Number | Description | Package Size |
|---|---|---|
| QG52906 | QIAGEN® QIAamp® Viral RNA Mini Kit (250) | 250 RNA preps |
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