QIAamp DNA Mini Kit provides silica-membrane-based nucleic acid purification from tissues, swabs, CSF, blood, body fluids or washed cells from urine. In addition, genomic and mitochondrial DNA can be purified from small amounts of fresh or frozen blood, tissue and dried blood spots. Mechanical homogenization is not required as the tissues are lysed enzymatically. Purification of DNA using the QIAamp DNA Micro Kit and QIAamp DNA Mini Kit is also automatable on the QIAcube Connect MDx.
QIAamp DNA Mini standard protocols can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson. The TRACKMAN Connected system guides researchers through the QIAamp DNA Mini protocols while automatically adjusting the Bluetooth-enabled PIPETMAN M Connected pipette settings. Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report.
Performance
DNA purified using the QIAamp DNA Micro Kit is free of proteins, nucleases and other impurities, and is suitable for use in sensitive downstream applications, such as real-time PCR and laser microdissection (LMD) PCR. Purified DNA may also be used in short-tandem repeat (STR) genotyping, single-nucleotide polymorphism (SNP) genotyping or pharmacogenomic research.
The QIAamp DNA Mini Kit yields DNA sized up to 50 kb. DNA of this length denatures completely and has the highest amplification efficiency. Yields of nucleic acids or DNA depend on the starting material. DNA purified using the QIAamp DNA Mini Kit can be used in a wide range of downstream applications, including PCR and quantitative real-time PCR, Southern blotting, SNP and STR genotyping and pharmacogenomic research.
The QIAamp DNA Mini QIAcube Kit yields DNA sized up to 50 kb: DNA of this length denatures completely and has the highest amplification efficiency. The purified DNA may be used in a number of applications, including:
- Viral testing and research
- Bacterial testing and research
- Fungal testing and research
- Cancer testing and research
- Human genetic testing research
- Paternity testing
- Forensic analysis
The QIAamp DNA Mini QIAcube Kit simplifies DNA isolation from tissue samples with fast spin-column procedures. The kit includes rotor adapters that are preloaded with QIAamp spin columns and elution tubes, delivering greater convenience and time savings.
Procedure
The purification procedure is designed to ensure safe and reproducible handling of The QIAamp DNA Micro Kit simplifies DNA isolation from small samples with a fast spin-column procedure. The QIAamp DNA Micro Kit uses well-established technology for purification of genomic and mitochondrial DNA from small sample volumes or sizes (e.g., 1–100 µl whole blood, blood cards of ~3 mm diameter, urine or <10 mg tissue or laser microdissected tissue). Fresh or frozen blood, tissue and dried blood spots can be purified in 30 minutes and eluted in 20–100 µl.
The QIAamp DNA Micro Kit can also be automated on the QIAcube Connect.
Optimized buffers and enzymes in the QIAamp DNA Mini Kit lyse samples, stabilize nucleic acids and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer.
No mechanical homogenization is necessary as the tissues are lysed enzymatically. The convenient spin-column procedure means that hands-on preparation time is only 20 minutes (lysis times differ according to the sample source). Samples can be processed using either a microcentrifuge or, if blood or other body fluids are being processed, using the QIAvac 24 Plus. In addition, the rigorous lysis procedure employed makes the QIAamp DNA Mini Kit ideal for purification of genomic DNA from bacteria or parasites. To further reduce hands-on time, genomic DNA purification may be automated on the QIAcube. The QIAamp DNA Accessory Set A provides the additional buffers and reagents needed for isolation of genomic, mitochondrial, bacterial, parasite or viral DNA using the QIAcube with 12 x QIAamp DNA Mini Kits (50).
Principle
The QIAamp DNA Micro Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 µl. QIAamp DNA Micro technology yields genomic and mitochondrial DNA from small samples ready to use in PCR and blotting procedures.
The QIAamp DNA Mini Kit simplifies DNA isolation from tissue samples with fast spin-column or vacuum procedures. QIAamp DNA technology yields genomic, mitochondrial, bacterial, parasite or viral DNA from human tissue samples ready to use in PCR and blotting procedures.
During the QIAamp DNA purification procedure, DNA binds specifically to the QIAamp MinElute or QIAamp silica-gel membrane while contaminants pass through. No phenol-chloroform extraction is required. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp DNA technology yields genomic, mitochondrial, bacterial, parasite or viral DNA from human tissue samples ready to use in PCR and blotting procedures.
QIAamp sample preparation technology is fully licensed, allowing QIAamp purified nucleic acids to be used in any molecular assay or other downstream application without risk of patent infringement.
Applications
The QIAamp DNA Micro Kit procedure is suitable for a wide range of sample materials, including small volumes of blood, blood cards, urine, small tissue samples, including laser microdissections.
The QIAamp DNA Mini Kit is ideal for purification of DNA from most commonly used human tissue samples, including muscle, liver, heart, brain, bone marrow and other tissues, swabs (buccal, eye, nasal, pharyngeal and others), CSF, blood, body fluids and washed cells from urine. DNA can be purified from up to 25 mg tissue or from up to 200 µl fluid in 20 minutes, and eluted in 50–200 µl.
The QIAamp DNA Mini QIAcube Kit is for automated isolation of genomic, mitochondrial, parasite or viral DNA on the QIAcube Connect. Sample sources include tissues, buccal swabs, CSF and washed cells from urine.
| Sample | Amount | Total nucleic acid yields (µg)* |
DNA yields (µg)†|
|---|---|---|---|
| Blood | 200 µl | 4–12 | 4–12 |
| Buffy coat | 200 µl | 25–50 | 25–50 |
| Cells | 106 | 20–30 | 15–20 |
| Liver | 25 mg | 60–115 | 10–30 |
| Brain | 25 mg | 35–60 | 15–30 |
| Lung | 25 mg | 25–45 | 5–10 |
| Heart | 25 mg | 15–40 | 5–10 |
| Kidney | 25 mg | 40–85 | 15–30 |
| Spleen | 10 mg | 25–45 | 5–30 |
Comparison of QIAamp DNA Kits
| Features | QIAamp DNA Micro Kit | QIAamp DNA Mini Kit |
|---|---|---|
| Applications | Real-time PCR, STR analysis, LMD-PCR | PCR, Southern blotting |
| Elution volume | 20–100 µl | 50–200 µl |
| Format | Spin column | Spin column |
| Main sample type | Whole blood | Whole blood, tissue, cells |
| Processing | Manual (centrifugation or vacuum) | Manual (centrifugation or vacuum) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein |
Genomic DNA, mitochondrial DNA | Genomic DNA, mitochondrial DNA, bacterial DNA, parasite DNA, viral DNA |
| Sample amount | 1–100 µl | 200 µl/25 mg/5 x 106 |
| Technology | Silica technology | Silica technology |
| Time per run or per prep | 30 minutes | 20 minutes |
| Yield | <3 µg | 4–30 µg |
FEATURES & BENEFITS
- Rapid purification of high-quality DNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Complete removal of contaminants and inhibitors
- Automated purification of 1–12 samples on the QIAcube Connect MDx
PRODUCT DETAILS
You may place order by giving us a call
| Catalog Number | Description | Units of measurement |
|---|---|---|
| QG51306 | QIAGEN® QIAamp DNA Mini Kit | 250 DNA preps |
