QIAamp DNA Mini Kit (250)

QIAamp DNA Mini Kit by Qiagen

QIAamp DNA Mini Kit is intended for isolation of genomic, mitochondrial, bacterial, parasite or viral DNA in molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

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Product Overview

The QIAamp® DNA Mini Kit (250) uses silica-membrane spin-column technology to purify genomic, mitochondrial, bacterial, parasite or viral DNA from a broad range of biological samples. Each kit provides components for 250 DNA preparations.

Optimized buffers and Proteinase K lyse samples and promote selective DNA binding to the QIAamp membrane. Contaminants and inhibitors are removed through two wash steps, and purified DNA is eluted in Buffer AE or water for use in downstream molecular biology applications.

The kit supports fresh or frozen blood, tissues, swabs, CSF, body fluids, cultured cells and other compatible sample types. Samples may be processed by microcentrifuge, by vacuum for supported sample types, or through automated purification on QIAcube® Connect.

For molecular biology applications. Not intended for the diagnosis, prevention or treatment of disease.

250 DNA Preps

Includes 250 QIAamp Mini Spin Columns, collection tubes, Proteinase K and purification buffers.

DNA up to 50 kb

Purifies DNA up to 50 kb in size, with fragments of approximately 20–30 kb predominating.

Flexible Processing

Process samples by centrifugation, supported vacuum protocols or QIAcube Connect automation.

Key Features

  • Rapid purification of high-quality, ready-to-use DNA
  • Purifies genomic, mitochondrial, bacterial, parasite and viral DNA
  • Silica-membrane spin-column technology
  • No phenol-chloroform extraction or alcohol precipitation
  • Two wash steps remove contaminants and PCR inhibitors
  • Consistent yields across a broad range of supported sample types
  • No mechanical homogenization required for standard tissue protocols because tissues are lysed enzymatically
  • Approximately 20 minutes of hands-on preparation; lysis time varies by sample source
  • Manual centrifugation, vacuum processing or QIAcube Connect automation
  • Purified DNA is suitable for PCR, quantitative real-time PCR, Southern blotting and genotyping applications

Product Specifications

Jant Catalog Number QG51306
QIAGEN Catalog Number 51306
Product Name QIAamp® DNA Mini Kit (250)
Purification Targets Genomic, mitochondrial, bacterial, parasite and viral DNA
Technology Silica-membrane technology
Format QIAamp Mini Spin Columns
Sample Input Up to 200 µL fluid, 25 mg tissue or 5 × 106 cells, depending on the protocol
Elution Volume 50–200 µL
DNA Size Up to 50 kb; approximately 20–30 kb predominating
Typical DNA Yield Approximately 4–30 µg, depending on sample type and starting material
Processing Manual centrifugation, supported vacuum protocols or QIAcube Connect automation
Hands-On Preparation Time Approximately 20 minutes; lysis time varies by sample source
Downstream Applications PCR, quantitative real-time PCR, Southern blotting, SNP genotyping, STR genotyping and pharmacogenomic research
Storage Store spin columns and buffers at 15–25°C. Proteinase K is stable for up to one year after delivery at room temperature; storage at 2–8°C is recommended to prolong its lifetime.
Intended Use Molecular biology applications; not intended for diagnosis, prevention or treatment of disease
Package Size 250 DNA preps

Typical DNA Yields by Sample Type

DNA yield depends on the sample type, sample condition and amount of starting material.

Sample Starting Amount Total Nucleic Acid Yield DNA Yield
Blood 200 µL 4–12 µg 4–12 µg
Buffy coat 200 µL 25–50 µg 25–50 µg
Cells 106 cells 20–30 µg 15–20 µg
Liver 25 mg 60–115 µg 10–30 µg
Brain 25 mg 35–60 µg 15–30 µg
Lung 25 mg 25–45 µg 5–10 µg
Heart 25 mg 15–40 µg 5–10 µg
Kidney 25 mg 40–85 µg 15–30 µg
Spleen 10 mg 25–45 µg 5–30 µg

Values are typical manufacturer-reported yields under the referenced conditions. Actual results vary. Refer to the current handbook for protocol details and information about RNase A treatment.

QIAamp DNA Micro Kit vs. QIAamp DNA Mini Kit

Both kits use silica-membrane spin columns, but they are designed for different sample amounts, yields and applications.

Feature QIAamp DNA Micro Kit QIAamp DNA Mini Kit
Applications Real-time PCR, STR analysis, LMD-PCR PCR, Southern blotting
Elution Volume 20–100 µL 50–200 µL
Format Spin column Spin column
Main Sample Types Whole blood and small samples Whole blood, tissue and cells
Processing Manual centrifugation or vacuum Manual centrifugation or vacuum
Purification Targets Genomic and mitochondrial DNA Genomic, mitochondrial, bacterial, parasite and viral DNA
Sample Amount 1–100 µL 200 µL fluid, 25 mg tissue or up to 5 × 106 cells
Technology Silica technology Silica technology
Preparation Time Approximately 30 minutes Approximately 20 minutes
Typical Yield Less than 3 µg 4–30 µg

This product page and the ordering information below are specifically for the QIAamp DNA Mini Kit (250). The QIAamp DNA Micro Kit is a separate product.

Kit Contents

  • 250 QIAamp Mini Spin Columns
  • 750 Collection Tubes (2 mL)
  • 2 × 33 mL Buffer AL
  • 50 mL Buffer ATL
  • 98 mL Buffer AW1 concentrate
  • 66 mL Buffer AW2 concentrate
  • 128 mL Buffer AE
  • 6 mL Proteinase K
  • Selection Guide
  • Components for 250 DNA preparations

How DNA Purification Works

The QIAamp DNA Mini procedure combines selective DNA binding on a silica membrane with spin, vacuum or automated processing.

  1. Lyse: Optimized buffers and Proteinase K lyse the sample and help stabilize nucleic acids.
  2. Bind: Alcohol is added and the lysate is loaded onto the spin column. DNA binds to the QIAamp silica membrane while many contaminants pass through.
  3. Wash: Buffer AW1 and Buffer AW2 remove residual contaminants and substances that can inhibit downstream enzymatic reactions.
  4. Elute: Purified DNA is eluted in Buffer AE or water for direct use in compatible downstream applications.

For standard tissue protocols, enzymatic lysis eliminates the need for mechanical homogenization. Purified DNA may be used immediately or stored according to the instructions in the current handbook.

Sample Types and Applications

Supported Sample Types

  • Fresh or frozen whole blood
  • Plasma, serum and buffy coat
  • Bone marrow and other body fluids
  • Cultured cells and lymphocytes
  • Fresh or frozen tissues
  • Dried blood spots
  • Buccal, eye, nasal and pharyngeal swabs
  • Cerebrospinal fluid
  • Washed cells from urine

Downstream Applications

  • PCR and quantitative real-time PCR
  • Southern blotting
  • SNP genotyping
  • STR genotyping
  • Pharmacogenomic research
  • Viral, bacterial and parasite DNA research
  • Other compatible molecular biology workflows

Sample preparation and protocol requirements vary. Refer to the current handbook before processing each sample type.

Processing and Automation Options

Spin-Column Processing

QIAamp Mini Spin Columns fit standard microcentrifuge workflows and support simultaneous processing of multiple samples.

Vacuum Processing

Supported blood and body-fluid protocols may be performed using the QIAvac 24 Plus and the required vacuum accessories.

QIAcube Connect Automation

Purification from blood, body fluids, tissues and bacteria can be automated on QIAcube Connect. Confirm the applicable protocol and accessory-set requirements before use.

Guided Digital Pipetting

QIAamp DNA Mini standard protocols can also be guided using the TRACKMAN Connected system with PIPETMAN M Connected pipettes, including automatic pipette-setting adjustments and run reporting.

Related Real-Time PCR Solutions

Purified DNA from the QIAamp® DNA Mini Kit can be used in compatible downstream PCR, qPCR and real-time PCR workflows. Explore Jant Pharmacal’s real-time PCR portfolio, including standard multiplex panels, lyophilized panels, custom pre-plated panels, RT-PCR analyzers and PGx testing options.

Explore Real-Time PCR Solutions

Review the requirements of the selected assay or panel to confirm sample, extraction and workflow compatibility.

Important Use and Safety Information

  • For molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of disease.
  • Follow appropriate laboratory procedures when handling chemicals and potentially infectious samples.
  • Buffers AL and AW1 contain guanidine hydrochloride. Do not add bleach or acidic solutions directly to sample-preparation waste.
  • Wear suitable protective clothing, disposable gloves and eye protection when working with kit chemicals.
  • Review the current handbook and applicable safety data sheets before use.

Documentation and Resources

Review the current manufacturer handbook and the protocol applicable to your sample type before using the kit.

QIAamp DNA Mini and Blood Mini Handbook QIAamp DNA Mini Kit Protocol Card QIAamp Genomic DNA Kits Product Profile View Product Information on QIAGEN

Frequently Asked Questions

The kit can purify genomic, mitochondrial, bacterial, parasite and viral DNA from supported sample types.

The QIAamp DNA Mini Kit (250) includes components for 250 DNA preparations.

Supported sample types include whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, cultured cells, tissues, dried blood spots, swabs, CSF and washed cells from urine. Use the protocol specific to the sample type.

Depending on the protocol, the kit can process up to 200 µL of fluid, 25 mg of tissue or 5 × 106 cells. Consult the handbook because input limits and preparation steps vary by sample type.

The manufacturer comparison lists a typical DNA yield range of approximately 4–30 µg for the QIAamp DNA Mini Kit. Actual yield depends on the sample type, amount and condition of the starting material.

The kit yields DNA up to 50 kb in size, with fragments of approximately 20–30 kb predominating.

Hands-on preparation takes approximately 20 minutes. Total processing time varies because lysis time depends on the sample source and selected protocol.

Mechanical homogenization is not required for the standard tissue protocols because tissues are lysed enzymatically. Follow any sample-specific preparation instructions in the handbook.

Yes. DNA purification from supported blood, body-fluid, tissue and bacterial samples can be automated on QIAcube Connect. Confirm the applicable protocol, accessory set and additional reagent requirements before use.

Store QIAamp Mini Spin Columns and buffers at 15–25°C. Proteinase K is stable for up to one year after delivery at room temperature; storage at 2–8°C is recommended to prolong its lifetime.

No. The kit is intended for molecular biology applications and is not intended for the diagnosis, prevention or treatment of disease.

Ordering Information

To place an order, call Jant Pharmacal at 800.676.5565

Catalog Number Description Package Size
QG51306 QIAGEN® QIAamp® DNA Mini Kit (250) 250 DNA preps

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