Mag-Bind® Blood & Tissue DNA HDQ 96 Kit: Genomic DNA Isolation From Blood and Tissue
The Mag-Bind® Blood & Tissue DNA HDQ 96 Kit has been designed to facilitate the isolation of top-tier genomic DNA from a diverse range of sources, including blood samples, saliva, swabs, mouse tails, dried blood spots, and cultured cells.
The Mag-Bind® Particles HDQ provides a rapid magnetic response time, which significantly minimizes the overall processing duration. This state-of-the-art system ingeniously combines the reversible nucleic acid-binding attributes of Mag-Bind® paramagnetic particles with Omega Bio-tek’s renowned tissue and blood DNA isolation mechanisms.
By harnessing the power of paramagnetic particles, it ensures the extraction of high quality DNA, which is perfectly tailored for immediate use in a vast array of downstream applications, such as Next Generation Sequencing, qPCR, PCR, and microarrays. The Mag-Bind® Blood & Tissue DNA HDQ 96 Kit can be scaled up to process 1 mL whole blood samples.
Protocols are available for the following automated platforms:
- Accuris IsoPUREâ„¢ 96
- Hamilton Microlab® STAR
- Hamilton Microlab® NIMBUS
- Tecan Fluent®
- Tecan Freedom EVO®
- Thermo Fisher Scientific KingFisher® Apex
- Thermo Fisher Scientific KingFisher® Flex
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Starting Amount | 100-250 μL blood samples, saliva, swabs, mouse tails, dried blood spots or 5 x 106 cultured cells. |
| Elution Volume | 50-200 µL |
| Technology | Magnetic Beads |
| Processing Mode | Automated, Manual |
| Throughput | 8 – 384 |
| Note | Downstream applications: NGS, qPCR, microarray |
KIT COMPONENTS
- Mag-Bind® Particles HDQ
- AL Buffer
- TL Buffer
- HDQ Binding Buffer
- VHB Buffer
- SPM Wash Buffer
- Proteinase K Solution
- Elution Buffer
Can the HDQ Binding Buffer and the Mag-Bind Particles HDQ be made into a master mix?
Yes, they can be made into a master mix before starting the experiment.
protocol for dried blood spots using the Mag-Bind Blood & Tissue DNA HDQ 96 Kit
- Cut or punch-out the blood spot from the filter paper (up to 200 µL blood can be used per spot). Tear or cut the filter paper into small pieces and place them into a 2-mL nuclease-free microcentrifuge tube.
- Add 250 µL PBS.
- Incubate at 65 °C for 1 hour. Vortex briefly every 20 minutes.
- Add 25 µL Proteinase K Solution. Vortex at maximum speed for 15 seconds.
- Incubate at 65 °C for 30 minutes. Vortex briefly several times during incubation.
- Centrifuge at ≥13,000 x g for 5 minutes.
- Transfer the supernatant to a nuclease-free 2-mL microcentrifuge tube.
- Add 290 µL AL Buffer and vortex, or pipet up and down 20 times, to mix. Optional: Add 5 µL RNase A to each sample. Vortex to mix. Let sit at room temperature for 2 minutes.
- Continue from step 5 (page 6) of the ‘Blood Protocol’ (250 µL) on the Mag-Bind Blood & Tissue DNA HDQ 96 Kit manual.
FEATURES & BENEFITS
- Safe – Free from Organic Solvents
- Compatible with DNA Genotek’s and Mawi iSWAB DNA Preservation System
- High quality – Purified DNA Suitable for Downstream Applications Such as NGS, qPCR, PCR, and microarrays
- Diverse Genomic DNA Isolation
- Rapid Magnetic Response
- Scalable Blood Sample Processing
PRODUCT DETAILS
You may place order by giving us a call
| Catalog Number | Description | Units of Measurements |
| M6399-00 | Mag-Bind® Blood & Tissue DNA HDQ 96 Kit | 1 x 96 preps |
| M6399-01 | Mag-Bind® Blood & Tissue DNA HDQ 96 Kit | 4 x 96 preps |
| M6399-02 | Mag-Bind® Blood & Tissue DNA HDQ 96 Kit | 24 x 96 preps |
DOCUMENTATION AND VIDEOS
Brochure, package inserts and additional literature provided below:
