Omega BioTek: Mag-Bind® TotalPure NGS

Mag-Bind® TotalPure NGS

Mag-Bind® TotalPure NGS: Clean-up and size selection of DNA and RNA for NGS workflows using magnetic beads. Mag-Bind® TotalPure NGS serves both manual and fully automated purification processes for DNA, RNA, and PCR products.

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Mag-Bind® TotalPure NGS | Size Selection Beads and PCR Clean Up

The Mag-Bind® TotalPure NGS is an efficient solution tailored for the purification of DNA and RNA in next-generation sequencing workflows, with remarkable recovery rates. This versatile product facilitates flexible size selection, accommodating left, right, or double-sided selection based on the chosen reagent-to-sample ratio. Mag-Bind® TotalPure NGS serves both manual and fully automated purification processes for DNA, RNA, and PCR products.

Utilizing Omega Bio-tek’s proprietary chemistries and the magnetic beads’ reversible nucleic acid-binding properties, this system selectively binds fragments, effectively removing excess nucleotides, primers, and non-targeted products like primer-dimers. The purified DNA and RNA are well-suited for various downstream applications, including NGS library preparation, microarrays, automated fluorescent sequencing, and restriction enzyme digestion.

Protocols are available for the following automated platforms:

  • Hamilton Microlab® STAR
  • Hamilton Microlab® NIMBUS
  • KingFisher™, BioSprint®, and MagMAX® 96

For Research Use Only. Not for use in diagnostic procedures.

FEATURES SPECIFICATIONS
Downstream application Cloning, NGS, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
Elution volume 15 µL or above
Starting material DNA or RNA: PCR products, gDNA, cDNA
Starting amount Scalable
DNA recovered >90% recovery for DNA >100 bp
Processing mode Automated; manual
Throughput 96-384 samples per run
DNA binding technology Magnetic beads
Storage 2°C – 8°C
Special note Size selection by varying beads ratio
Can Mag-Bind TotalPure NGS be used for “High Molecular Weight (HMW)” DNA?

Mag-Bind TotalPure NGS can be used to clean HMW gDNA. We have cleaned up fragments as large as 50-60 kb with good efficiency.

Can Mag-Bind TotalPure NGS be used for DNA and RNA?

Yes. DNA and RNA can be cleaned up using Mag-Bind TotalPure NG

What is the smallest size recovery using TotalPure NGS?

Recovery success has been achieved for fragments down to 100 bp. Moreover, a protocol modification involving the addition of 1.8 volumes of beads and 1 volume of isopropyl alcohol has resulted in successful recovery of fragments as short as 50 bp. Please add 1.8 volumes of beads and 1 volume of isopropyl alcohol.

What is the difference between TotalPure NGS and RxnPure Plus?
TotalPure NGS has a much quicker magnetization time than the RxnPure particles, and TotalPure NGS particles are produced and quality controlled in an RNase-free environment allowing them to be used in RNA clean-up applications.

FEATURES & BENEFITS

  • No protocol change against major competitor
  • NGS:  Double or single-sided size selection
  • DNA cleanup: PCR cleanup
  • RNA cleanup: cDNA or RNA purification
  • Manual or adaptable to most open-ended liquid handlers
  • Significant cost savings
  • 96- or 384-well formats

PRODUCT DETAILS

You may place order by giving us a call

Catalog Number Description Units of Measurements
M1378-00 Mag-Bind® TotalPure NGS 5 mL
M1378-01 Mag-Bind® TotalPure NGS 50 mL
M1378-02 Mag-Bind® TotalPure NGS 500 mL

DOCUMENTATION AND VIDEOS

Brochure, package inserts and additional literature provided below:

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