Omega Bio-tek Mag-Bind® TotalPure NGS

Omega Bio-tek Mag-Bind TotalPure NGS

Mag-Bind® TotalPure NGS is an RNase-free magnetic-bead reagent for DNA and RNA cleanup, PCR-product purification and single- or double-sided size selection in next-generation sequencing workflows. The scalable system supports manual processing and automation on compatible open liquid handlers and magnetic processors. Available in 5 mL, 50 mL and 500 mL volumes. For Research Use Only. Not for use in diagnostic procedures.

Request Info Request Quote
  • This field is for validation purposes and should be left unchanged.
  • This field is hidden when viewing the form
  • This field is hidden when viewing the form
  • This field is hidden when viewing the form
    Leave the box checked if you wish to receive occasional special offer emails, or uncheck it to decline. Don't worry, we won't spam you.
  • This field is hidden when viewing the form
  • This field is hidden when viewing the form
  • This field is hidden when viewing the form
    Leave the box checked if you wish to receive occasional special offer emails, or uncheck it to decline. Don't worry, we won't spam you.

Product Overview

Omega Bio-tek Mag-Bind® TotalPure NGS is a magnetic-bead reagent for cleanup and size selection of DNA and RNA used in next-generation sequencing and other molecular research workflows. The reversible nucleic-acid binding chemistry can be used to purify PCR products, genomic DNA, cDNA and RNA while removing excess nucleotides, primers and smaller non-target products such as primer-dimers.

Fragment recovery is controlled by the selected bead-to-sample ratio, giving researchers flexibility for single-sided or double-sided size selection. The reagent is produced and quality controlled in an RNase-free environment for RNA cleanup applications. Workflows can be performed manually or adapted to compatible open liquid handlers and magnetic processors.

For Research Use Only. Not for use in diagnostic procedures.

DNA and RNA Cleanup

Supports PCR products, genomic DNA, cDNA and RNA cleanup for research workflows.

Flexible Size Selection

Use protocol-defined bead ratios for left-sided, right-sided or double-sided selection.

Manual or Automated

Scalable magnetic-bead processing for 96- or 384-well research workflows.

Key Features

  • Magnetic-bead cleanup and size selection for DNA and RNA
  • Designed for next-generation sequencing library-preparation workflows
  • Supports PCR-product cleanup and purification of genomic DNA, cDNA and RNA
  • Single-sided or double-sided size selection based on the selected bead-to-sample ratio
  • Manufacturer specification of >90% recovery for DNA fragments larger than 100 bp
  • Produced and quality controlled in an RNase-free environment for RNA cleanup applications
  • Manual processing or automation on compatible open liquid handlers and magnetic processors
  • Scalable starting amount with 96- and 384-well workflow support
  • Manufacturer-listed elution volume of 15 µL or greater; use the volume specified by the applicable protocol
  • Purified nucleic acids can support compatible research applications including NGS, PCR, qPCR, sequencing, cloning, microarrays, nucleic-acid labeling, in vitro transcription, restriction digestion and Southern blotting
  • Available in 5 mL, 50 mL and 500 mL volumes
  • Store at 2–8°C

Recovery, fragment cutoff and yield depend on nucleic-acid type, input, bead ratio, protocol, automation setup and elution conditions.

Cleanup and Size-Selection Workflow

The exact bead ratio, sample volume, incubation time, wash volume and elution conditions must follow the applicable library-preparation method or validated laboratory protocol.

1. Configure

Determine the bead-to-sample ratio required for cleanup or the desired fragment-size selection.

2. Bind

Resuspend the beads, combine them with the sample, mix and incubate as directed by the protocol.

3. Wash and Dry

Magnetize the particles, remove the designated fraction and wash the beads twice with 70% ethanol before drying.

4. Elute

Resuspend the dried beads in the specified elution solution, magnetize and transfer the cleared eluate.

Product Specifications

Product Mag-Bind® TotalPure NGS
Manufacturer Omega Bio-tek
Product Family M1378
Technology Reversible nucleic-acid binding with magnetic particles
Starting Materials DNA or RNA, including PCR products, genomic DNA and cDNA
Starting Amount Scalable
Fragment Selection Single-sided or double-sided selection by varying the bead-to-sample ratio
DNA Recovery Manufacturer specification: >90% recovery for DNA fragments >100 bp
Elution Volume 15 µL or greater; protocol-specific volumes apply
Processing Manual or automated
Listed Throughput 96–384 samples per run
Plate Formats 96-well and 384-well protocols available
Storage 2–8°C
Available Volumes 5 mL, 50 mL and 500 mL
Regulatory Designation For Research Use Only. Not for use in diagnostic procedures.

Components and Workflow Requirements

Mag-Bind TotalPure NGS is supplied as a bead reagent. The processing plates, magnetic device and other workflow materials are supplied by the laboratory.

Supplied Product

  • Mag-Bind® TotalPure NGS magnetic-bead reagent
  • 5 mL, 50 mL or 500 mL volume, according to catalog number
  • User manual

Supplied by the Laboratory

  • Compatible 96- or 384-well sample and elution plates
  • Compatible magnetic separation device
  • Vortex mixer
  • Multichannel pipettor and disposable reservoirs
  • Plate-sealing film
  • Freshly prepared 70% ethanol
  • Elution Buffer, TE Buffer or nuclease-free water, as specified by the protocol
  • RNase-free consumables and handling practices for RNA workflows

Manual and Automated NGS Cleanup

Mag-Bind TotalPure NGS can be processed manually with a compatible magnetic separation device or adapted to open liquid-handling platforms and magnetic processors. Jant-hosted product literature includes workflows or supporting data for 96- and 384-well manual processing, the Hamilton Microlab® STAR™, and the Tecan Fluent® 780. The current Jant listing also identifies protocol availability for Hamilton Microlab STAR and NIMBUS, KingFisher™, BioSprint® and MagMAX® 96 platforms.

Automation compatibility is configuration- and protocol-specific. Confirm the instrument model, plate type, deck layout, magnetic device, bead and sample volumes, mixing, incubation, wash, drying and elution settings before implementation.

Research Applications

  • NGS library cleanup and fragment-size selection
  • PCR-product cleanup
  • Genomic DNA and cDNA cleanup
  • RNA and in vitro transcribed RNA cleanup
  • Microarrays and automated fluorescent sequencing
  • Restriction-enzyme digestion
  • Cloning, PCR and real-time quantitative PCR
  • Nucleic-acid labeling and Southern blotting

Important Product Information

  • This product is for research use only and is not intended for diagnostic procedures.
  • There is no universal bead-to-sample ratio. Use the ratio specified by the applicable NGS library-preparation method or validated cleanup protocol.
  • Increasing the bead ratio generally permits smaller fragments to bind; using less reagent excludes more small fragments. Exact cutoffs depend on the sample and workflow.
  • The published >90% DNA-recovery specification applies to DNA fragments larger than 100 bp. Recovery varies with input, fragment size, bead ratio and processing conditions.
  • Omega Bio-tek reports successful routine recovery down to approximately 100 bp. Recovery near 50 bp requires a protocol modification and must not be assumed from the standard protocol.
  • The reagent can be used to clean high-molecular-weight genomic DNA, but efficiency and integrity should be evaluated for the laboratory’s specific fragment range and workflow.
  • Thoroughly resuspend the magnetic particles and allow the reagent to reach room temperature before use.
  • Residual ethanol can interfere with downstream applications. Follow the prescribed wash and drying conditions.
  • The Jant-hosted user manual is dated November 2018, revision v2.1, and the quick guide is revision 1.1. Confirm whether newer manufacturer documentation applies before beginning work.
  • Confirm current product availability, storage requirements and automation support when requesting a quote.

NGS Workflow Support

Explore related Jant resources for NGS sample preparation, library quality control and broader sequencing workflows.

NGS Extraction and Sample Preparation NGS Quality Control Explore Next-Generation Sequencing

Documentation and Resources

Review the full protocol and the applicable library-preparation instructions before selecting bead ratios or processing samples.

Product Literature

Mag-Bind TotalPure NGS Brochure Mag-Bind TotalPure NGS Protocol Mag-Bind TotalPure NGS Quick Guide

Technical References and Application Notes

DNA Size Selection Technical Reference Automated DNA Cleanup Application Note In Vitro Transcribed RNA Cleanup Application Note

Frequently Asked Questions

Mag-Bind TotalPure NGS is used for magnetic-bead cleanup and size selection of DNA and RNA in next-generation sequencing and compatible molecular research workflows. Applications include NGS library cleanup, PCR-product purification, genomic DNA and cDNA cleanup, and RNA purification.

Yes. Omega Bio-tek lists DNA and RNA cleanup, including PCR products, genomic DNA, cDNA and RNA. The particles are produced and quality controlled in an RNase-free environment for RNA cleanup applications.

The bead-to-sample ratio controls which fragment sizes bind to the magnetic particles. Higher bead volumes generally allow smaller fragments to bind, while lower bead volumes exclude more small fragments. Use the ratio specified by the library-preparation method or validated laboratory protocol.

Omega Bio-tek lists greater than 90% recovery for DNA fragments larger than 100 bp. Actual recovery depends on sample input, fragment distribution, bead ratio, mixing, magnetic separation, wash, drying and elution conditions.

Omega Bio-tek reports successful recovery down to approximately 100 bp with the standard workflow. The manufacturer also describes some recovery near 50 bp using a modified protocol with additional bead reagent and isopropanol. Follow the current manufacturer protocol and validate the required fragment range.

Omega Bio-tek reports using Mag-Bind TotalPure NGS to clean high-molecular-weight genomic DNA fragments as large as approximately 50–60 kb with good efficiency. Performance should be evaluated for the laboratory’s specific input and downstream requirements.

Yes. The reagent can be processed manually or adapted to compatible open liquid handlers and magnetic processors. Instrument-specific deck layouts, plate formats, volumes, mixing and timing must be confirmed and validated for the selected platform.

The current manufacturer specification lists an elution volume of 15 µL or greater. The Jant-hosted 96-well protocol uses 30–40 µL and the 384-well protocol uses 30 µL. Follow the volume specified by the applicable protocol and downstream workflow.

Store the reagent at 2–8°C. Allow it to reach room temperature and thoroughly resuspend the magnetic particles before use according to the protocol.

Mag-Bind TotalPure NGS is available as M1378-00, 5 mL; M1378-01, 50 mL; and M1378-02, 500 mL.

No. Mag-Bind TotalPure NGS is for research use only and is not for use in diagnostic procedures.

Ordering Information

To confirm availability or request pricing, call Jant Pharmacal at 800.676.5565

Catalog Number Description Unit of Measurement
M1378-00 Mag-Bind® TotalPure NGS 5 mL
M1378-01 Mag-Bind® TotalPure NGS 50 mL
M1378-02 Mag-Bind® TotalPure NGS 500 mL