Mag-Bind® TotalPure NGS | Size Selection Beads and PCR Clean Up
The Mag-Bind® TotalPure NGS is an efficient solution tailored for the purification of DNA and RNA in next-generation sequencing workflows, with remarkable recovery rates. This versatile product facilitates flexible size selection, accommodating left, right, or double-sided selection based on the chosen reagent-to-sample ratio. Mag-Bind® TotalPure NGS serves both manual and fully automated purification processes for DNA, RNA, and PCR products.
Utilizing Omega Bio-tek’s proprietary chemistries and the magnetic beads’ reversible nucleic acid-binding properties, this system selectively binds fragments, effectively removing excess nucleotides, primers, and non-targeted products like primer-dimers. The purified DNA and RNA are well-suited for various downstream applications, including NGS library preparation, microarrays, automated fluorescent sequencing, and restriction enzyme digestion.
Protocols are available for the following automated platforms:
- Hamilton Microlab® STAR
- Hamilton Microlab® NIMBUS
- KingFisher™, BioSprint®, and MagMAX® 96
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream application | Cloning, NGS, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting |
| Elution volume | 15 µL or above |
| Starting material | DNA or RNA: PCR products, gDNA, cDNA |
| Starting amount | Scalable |
| DNA recovered | >90% recovery for DNA >100 bp |
| Processing mode | Automated; manual |
| Throughput | 96-384 samples per run |
| DNA binding technology | Magnetic beads |
| Storage | 2°C – 8°C |
| Special note | Size selection by varying beads ratio |
Can Mag-Bind TotalPure NGS be used for “High Molecular Weight (HMW)” DNA?
Mag-Bind TotalPure NGS can be used to clean HMW gDNA. We have cleaned up fragments as large as 50-60 kb with good efficiency.
Can Mag-Bind TotalPure NGS be used for DNA and RNA?
Yes. DNA and RNA can be cleaned up using Mag-Bind TotalPure NG
What is the smallest size recovery using TotalPure NGS?
Recovery success has been achieved for fragments down to 100 bp. Moreover, a protocol modification involving the addition of 1.8 volumes of beads and 1 volume of isopropyl alcohol has resulted in successful recovery of fragments as short as 50 bp. Please add 1.8 volumes of beads and 1 volume of isopropyl alcohol.
What is the difference between TotalPure NGS and RxnPure Plus?
FEATURES & BENEFITS
- No protocol change against major competitor
- NGS: Double or single-sided size selection
- DNA cleanup: PCR cleanup
- RNA cleanup: cDNA or RNA purification
- Manual or adaptable to most open-ended liquid handlers
- Significant cost savings
- 96- or 384-well formats
PRODUCT DETAILS
You may place order by giving us a call
| Catalog Number | Description | Units of Measurements |
| M1378-00 | Mag-Bind® TotalPure NGS | 5 mL |
| M1378-01 | Mag-Bind® TotalPure NGS | 50 mL |
| M1378-02 | Mag-Bind® TotalPure NGS | 500 mL |
DOCUMENTATION AND VIDEOS
Brochure, package inserts and additional literature provided below:
