Standard Multiplex RT-PCR Panel
Accutest® Standard Wound RT-PCR Panel
A predefined multiplex Research Use Only panel featuring 24 organism targets plus RNase P (RPP30) as the internal control.
The Standard Wound menu includes Gram-negative bacteria, Gram-positive bacteria and selected Candida species for molecular wound research workflows.
Organism Targets
A predefined bacterial and Candida research menu.
Plate Formats
Pre-spotted plate configurations plus a tube option.
MasterMix
MasterMix is included with the panel mixes.
Target Menu
Standard Wound Assay Menu
The panel includes 24 organism targets.
RNase P (RPP30) is included separately as the internal control,
for 25 total listed entries.
Gram-Negative Bacteria
- Acinetobacter baumannii
- Bacteroides fragilis
- Citrobacter freundii
- Citrobacter koseri
- Enterobacter cloacae
- Escherichia coli
- Klebsiella (Enterobacter) aerogenes
- Klebsiella pneumoniae / oxytoca
- Morganella morganii
- Proteus mirabilis
- Proteus vulgaris
- Pseudomonas aeruginosa
- Serratia marcescens
Gram-Positive & Additional Bacteria
- Enterococcus faecalis
- Enterococcus faecium
- Staphylococcus aureus
- Staphylococcus epidermidis
- Staphylococcus saprophyticus
- Streptococcus agalactiae
- Streptococcus pyogenes
- Ureaplasma urealyticum
Candida Targets
- Candida albicans
- Candida glabrata
- Candida parapsilosis
Internal Control:
RNase P (RPP30) is included as the panel’s internal control.
Flexible Configuration
Choose the Format for Your Workflow
The Standard Wound panel is available in pre-spotted plate and tube
configurations for different molecular research workflows.
Pre-Spotted Plate Formats
- 96-well plate configuration available
- 384-well plate — clear
- 384-well plate — white
- Primers and probes pre-spotted in plate configurations
Tube Configuration
- 100-reaction tube format
- MasterMix included with panel mixes
- Alternative to the pre-spotted plate configuration
Technical Information
Panel Specifications
Review the standard configuration below and confirm instrument-specific
requirements with Jant before ordering.
| Specification | Standard Wound Panel |
|---|---|
| Organism Targets | 24 |
| Total Listed Entries | 25 including RNase P (RPP30) |
| Internal Control | RNase P (RPP30) |
| MasterMix | Included |
| Plate Formats | 96-well and 384-well |
| Tube Format | 100 reactions |
| 96-Well Reaction Volume | 20 µL |
| 384-Well Reaction Volume | 10 µL |
| Cycling | Standard and fast cycling protocols supported |
| Shipping | Dry ice |
| Storage | –15°C to –25°C |
| Use | Research Use Only |
Workflow
Standard RT-PCR Setup
Final setup should follow the current product documentation and
instrument-specific requirements.
Prepare the Reaction
Prepare the included MasterMix according to the applicable panel instructions
and selected configuration.
Add Template
Add the appropriate nucleic-acid template according to the current research
workflow and product instructions.
Seal & Prepare
Seal the selected reaction vessel, mix as required and prepare it
for amplification.
Run RT-PCR
Run the appropriate cycling protocol on a compatible real-time PCR instrument.
Ordering Information
Available Configurations
The catalog numbers below are currently confirmed on the JantDX product listing.
Contact Jant for the appropriate 96-well catalog number.
| Catalog Number | Format |
|---|---|
| WOU-01-C-384 | Pre-spotted 384-well plate, clear |
| WOU-01-W-384 | Pre-spotted 384-well plate, white |
| WOU-01-T-384-100R | Tube format, 100 reactions |
Before ordering:
Confirm the plate or tube format, instrument model, fluorescence channels
and any additional control requirements with Jant Pharmacal.
Standard or Custom Wound Panel?
Choose the predefined Standard Wound menu or a custom configuration
based on the targets required for your research workflow.
Standard Wound Panel
Choose the Standard panel when the predefined 24-organism menu matches
your research requirements.
- 24 organism targets
- RNase P (RPP30) internal control
- Pre-spotted plate configurations
- 100-reaction tube format
- MasterMix included
Custom Pre-Plated Wound Panel
For research workflows requiring a different combination of
available wound-related assays.
Frequently Asked Questions
Standard Wound RT-PCR Panel FAQ
How many targets are included?
The Standard Wound panel includes 24 organism targets plus RNase P (RPP30)
as the internal control, for 25 total listed entries.
What types of organisms are represented?
The predefined menu includes Gram-negative bacteria, Gram-positive bacteria
and selected Candida species.
Is RNase P included?
Yes. RNase P (RPP30) is included as the panel’s internal control.
Is MasterMix included?
Yes. MasterMix is included with the panel mixes.
Which formats are available?
The Standard Wound category states that 96-well, 384-well and tube formats
are available. The current product listing publishes catalog numbers for the
clear and white 384-well plates and the 100-reaction tube configuration.
Contact Jant to confirm the appropriate 96-well catalog number.
What reaction volumes are used?
The standard configuration uses a 20 µL reaction volume for 96-well formats
and a 10 µL reaction volume for 384-well formats.
Are standard and fast cycling protocols supported?
Yes. Standard and fast cycling protocols are supported. Confirm the appropriate
protocol for the selected configuration and instrument before use.
How is the panel shipped and stored?
The panel is shipped on dry ice and should be stored at –15°C to –25°C.
Is this panel intended for diagnostic use?
No. The Accutest® Standard Wound RT-PCR Panel is offered for Research Use Only
and is not for use in diagnostic procedures.
Request Information
Need Help Selecting a Wound RT-PCR Configuration?
Complete the form below and Jant Pharmacal can help review the appropriate
format and instrument requirements for your research workflow.
You can also call
800.676.5565.