Formalin-fixed, paraffin-embedded (FFPE) tissue is widely used in cancer research and molecular oncology. Pathology archives provide valuable specimens for studying genetic alterations, but preparing these samples for next-generation sequencing (NGS) presents unique challenges. The same formalin fixation process that preserves tissue morphology can also fragment and chemically modify DNA and RNA. These changes may affect […]
Every laboratory running real-time PCR eventually encounters unexpected results: a flat amplification curve, Ct values that shift later than expected, or amplification in a no-template control (NTC). These problems can originate from sample quality, extraction, reagents, assay setup, or instrument settings. Identifying the cause requires a systematic approach rather than changing multiple variables at once. […]
When a real-time PCR run produces unexpectedly high Ct values or an NGS library fails quality control, the problem may start before amplification or sequencing—with nucleic acid extraction. DNA and RNA extraction is a critical step in molecular workflows. Poor recovery, contamination, or inhibitor carryover can affect downstream results, even when assays and instruments perform […]
RUO, IVD and LDT describe different concepts in laboratory testing. Learn what each term means, how they differ, and what laboratories should consider when evaluating molecular products.
Real-time PCR and next-generation sequencing answer different molecular questions. Compare target coverage, throughput, workflow complexity, data analysis and other considerations when choosing an approach for your laboratory.
The 2026–27 respiratory season is beginning. See how multiplex RT-PCR panels, extraction workflows and thoughtful target selection can help molecular laboratories prepare for changing respiratory research demands.