Formalin-fixed, paraffin-embedded (FFPE) tissue is widely used in cancer research and molecular oncology. Pathology archives provide valuable specimens for studying genetic alterations, but preparing these samples for next-generation sequencing (NGS) presents unique challenges. The same formalin fixation process that preserves tissue morphology can also fragment and chemically modify DNA and RNA. These changes may affect […]
Every laboratory running real-time PCR eventually encounters unexpected results: a flat amplification curve, Ct values that shift later than expected, or amplification in a no-template control (NTC). These problems can originate from sample quality, extraction, reagents, assay setup, or instrument settings. Identifying the cause requires a systematic approach rather than changing multiple variables at once. […]
When a real-time PCR run produces unexpectedly high Ct values or an NGS library fails quality control, the problem may start before amplification or sequencing—with nucleic acid extraction. DNA and RNA extraction is a critical step in molecular workflows. Poor recovery, contamination, or inhibitor carryover can affect downstream results, even when assays and instruments perform […]
RUO, IVD and LDT describe different concepts in laboratory testing. Learn what each term means, how they differ, and what laboratories should consider when evaluating molecular products.
Real-time PCR and next-generation sequencing answer different molecular questions. Compare target coverage, throughput, workflow complexity, data analysis and other considerations when choosing an approach for your laboratory.
The 2026–27 respiratory season is beginning. See how multiplex RT-PCR panels, extraction workflows and thoughtful target selection can help molecular laboratories prepare for changing respiratory research demands.
Wound and surgical site infections impact recovery and care. Learn key causes, risk factors, and modern tools that improve detection.
Urinary tract infections (UTIs) are among the most common bacterial infections, affecting millions of people each year in the United States. While they can occur in anyone, women are particularly susceptible due to anatomical differences, such as a shorter urethra that allows bacteria to reach the bladder more easily. Men, older adults, and individuals with […]
Sexually transmitted infections (STIs) are increasing at an alarming rate worldwide, posing significant public health concerns. Recent data shows that STI cases have surged in many regions, including the Americas and Africa1. Factors such as changing sexual behaviors, decreased condom use, and reduced access to healthcare services contribute to this rise. The increase underscores the […]
Antibiotic resistance is one of the most significant public health threats today. It occurs when bacteria evolve and become resistant to antibiotics that once killed them. This resistance develops due to the overuse and misuse of antibiotics, allowing bacteria to survive and multiply despite treatment. How Does Antibiotic Resistance Happen? Bacteria naturally adapt to their […]