Every laboratory running real-time PCR eventually encounters unexpected results: a flat amplification curve, Ct values that shift later than expected, or amplification in a no-template control (NTC). These problems can originate from sample quality, extraction, reagents, assay setup, or instrument settings. Identifying the cause requires a systematic approach rather than changing multiple variables at once. […]
When a real-time PCR run produces unexpectedly high Ct values or an NGS library fails quality control, the problem may start before amplification or sequencing—with nucleic acid extraction. DNA and RNA extraction is a critical step in molecular workflows. Poor recovery, contamination, or inhibitor carryover can affect downstream results, even when assays and instruments perform […]
Real-time PCR and next-generation sequencing answer different molecular questions. Compare target coverage, throughput, workflow complexity, data analysis and other considerations when choosing an approach for your laboratory.
qPCR is a molecular biology technique used to amplify and quantify DNA or RNA in a biological sample.
Multiplex Vs Monoplex comparison. What are the advantages and disadvantages of each? Learn about single and multi panels in RT-PCR.
Real-Time Polymerase Chain Reaction (real-time PCR) has revolutionized the diagnosis and management of infectious diseases in acute care settings. Through the coupling of the amplification and direct detection of target microbial DNA in a single reaction vessel, real-time PCR drastically reduces the need for microbial culture methods, decreases wait times required for pathogen detection, and […]