The Accutest Standard UTI RT-PCR Panel provides a comprehensive, multiplexed real-time PCR solution for the detection of urinary tract infection (UTI) pathogens. Each panel includes optimized primers and probes for 24 clinically relevant targets, covering more than 95% of the most prevalent clinical UTI cases. An internal control is incorporated in every reaction to validate nucleic acid extraction, amplification, and detection, ensuring high confidence and reproducibility across assays.

The panel is available in two formats: pre-spotted 384-well plates for high-throughput testing and tubes containing sufficient reagents for 100 reactions. This dual offering provides flexibility for laboratories to select the configuration best suited to their workflow and throughput requirements.

By consolidating multiple pathogen detections into a single multiplex reaction, the Accutest UTI RT-PCR Panel reduces turnaround time and hands-on labor compared to culture-based methods, while conserving reagents and improving efficiency. The pre-spotted plate design eliminates manual primer and probe preparation, reducing the risk of contamination and pipetting variability. All products are shipped on dry ice and stored at –15 °C to –25 °C, ensuring long-term stability and consistent lot-to-lot performance.

Sample input may include direct specimens or purified nucleic acids, with reactions prepared in a 10 μL final volume. Both standard and fast cycling protocols are supported, allowing laboratories to balance sensitivity and run time according to research needs.

The RT-PCR panel is intended for research use only and is not validated for diagnostic procedures. With broad pathogen coverage and a streamlined operational workflow, it provides a robust and scalable platform for molecular UTI research.

This multiplex assay is designed to detect and differentiate a broad range of clinically relevant targets associated with UTI studies, combining analytical precision with workflow efficiency. Available in 384-well pre-spotted plates and tube formats (100 reactions), the panel supports flexible throughput and consistent performance across molecular research environments.

Broad-Range 4X MasterMix is a versatile one-step RT-PCR solution optimized for multiplex applications, offering fast, reliable performance and seamless compatibility with all Accutest RT-PCR panels.

Composition and Features

• Integrated enzymes: Hot-start reverse transcriptase and hot-start DNA polymerase for efficient cDNA synthesis and amplification.
• Balanced reaction mix: Includes optimized dNTPs, MgCl₂, proprietary enhancers, and stabilizers to maximize performance under multiplex conditions.
• One-step RT-PCR: Built-in reverse transcription reduces run time and simplifies detection of single-stranded RNA viruses.

Advantages

• High performance across multiplex RT-PCR panels
• Reliable amplification from challenging sample matrices
• Reduced assay time with integrated reverse transcription
• Universal compatibility with Accutest RT-PCR panel designs
• Flexible use across diverse workflows