VIASURE SARS-CoV-2 Real Time PCR Detection Kit is designed for the specific identification and differentiation of 2019 Novel Coronavirus (SARS-CoV-2) in respiratory samples from patients with signs and symptoms of COVID-19 infection. This test is intended to be used for research purposes, without any medical objective is not regarded as devices for performance evaluation. RNA is extracted from respiratory specimens, amplified using RT-PCR and detected using fluorescent reporter dye probes specific for SARS-CoV-2.
Coronavirus are enveloped non-segmented positive-sense RNA viruses and belong to Coronaviridae family. There are six coronavirus species known to cause human diseases. Four viruses (229E, OC43, NL63 and HKU1) cause common cold symptoms and the other two (severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV)) are zoonotic and producing more severe complications. SARS-CoV and MERS-CoV have caused more than 10,000 cumulative cases in the past two decades, with mortality rates of 34% MERS-CoV and 10% SARS-CoV.
It is designed for the identification of SARS-CoV-2 in respiratory samples. The detection is done in one step real time RT format where the reverse transcription and the subsequent amplification of specific target sequence occur in the same reaction well. The isolated RNA target is transcribed generating complementary DNA by reverse transcriptase which is followed by the amplification of a conserved region of ORF1ab and N genes for SARS-CoV-2 using specific primers and a fluorescent-labeled probe.
The SARS-CoV-2 Detection Kit is based on the 5´ exonuclease activity of DNA polymerase. During DNA amplification, this enzyme cleaves the probe bounded to the complementary DNA sequence, separating the quencher dye from the reporter. This reaction generates an increase in the fluorescent signal which is proportional to the quantity of target template. This fluorescence can be measured on Real Time PCR platforms.
Available in strip or tube format.
Learn more about SARS-CoV-2 from the CDC.
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|JTVSNCO296TRUO||VIASURE SARS-CoV-2 Real Time PCR Detection Kit||4 tubes x 24 rxn|
|JTVSNCO206LRUO||VIASURE SARS-CoV-2 Real Time PCR Detection Kit||6 x 8-well strips, low profile|
|JTVSNCO206HRUO||VIASURE SARS-CoV-2 Real Time PCR Detection Kit||6 x 8-well strips, high profile|
|JTVSNCO212LRUO||VIASURE SARS-CoV-2 Real Time PCR Detection Kit||12 x 8-well strips, low profile|
|JTVSNCO212HRUO||VIASURE SARS-CoV-2 Real Time PCR Detection Kit||12 x 8-well strips, high profile|