The QIAamp DNA FFPE Tissue Kit is specially designed for purifying DNA from formalin-fixed, paraffin-embedded tissue sections. It uses special lysis conditions to release DNA from tissue sections and to overcome inhibitory effects caused by formalin crosslinking of nucleic acids. The kit uses QIAamp MinElute spin columns for purification of high-quality DNA in small volumes. Purification of DNA using this kit can be automated on the QIAcube Connect MDx.
The QIAamp DNA FFPE Tissue Kit purifies DNA that can be used in a wide range of downstream applications, such as single nucleotide polymorphism (SNP) and short-tandem repeat (STR) genotyping and pharmacogenomic research. DNA purified with the kit can also be used in PCR.
The QIAamp DNA FFPE Tissue procedure consists of 6 steps: remove paraffin, lyse, heat, bind, wash, and elute. After sample lysis, the procedure, which is highly suited for simultaneous processing of multiple samples, yields pure DNA in less than 30 minutes.
The QIAamp DNA FFPE Tissue Kit uses well-established QIAamp MinElute technology for purification of genomic and mitochondrial DNA from small sample volumes or sizes. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 μl.
Specially optimized lysis conditions allow genomic DNA to be efficiently purified from FFPE tissue sections without the need for overnight incubation. Incubation at an elevated temperature after proteinase K digestion partially removes formalin crosslinking of the released DNA, improving yield as well as DNA performance in downstream assays. Note that DNA isolated from FFPE samples is usually of lower molecular weight than DNA from fresh or frozen samples. The degree of fragmentation depends on the type and age of the sample and the conditions used for fixation.
|Applications||Real-time PCR, STR analysis, LMD-PCR|
|Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein||Genomic DNA, mitochondrial DNA|
|Elution volume||20-100 µl|
|Main sample type||Formalin fixed paraffin embedded tissue samples|
|Sample amount||Up to 8 sections, each with a thickness of up to 10 µm and a surface area of up to 250 mm2|
FEATURES & BENEFITS
- Rapid purification of high-quality, ready-to-use DNA
- Consistent, high yields
- Complete removal of contaminants and inhibitors
You may place order by giving us a call
|Catalog Number||Description||Units of measurement|
|QG56404||QIAGEN® QIAamp DNA FFPE Tissue Kit||50 DNA preps|