Product Description
The C. DIFF QUIK CHEK ® test is a rapid membrane enzyme immunoassay for use as a screening test to detect Clostridium difficile antigen, glutamate dehydrogenase (GDH), in fecal specimens from persons suspected of having C. difficile disease.
The test does not distinguish toxigenic from nontoxigenic strains of C. difficile.
The device contains a Reaction Window with two stripes.
One stripe is a test line specific for GDH, and the other is the control line.
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C. DIFF QUIK CHEK:
The C. DIFF QUIK CHEK test uses antibodies specific for the glutamate dehydrogenase of C. difficile. The device contains a Reaction Window with two vertical lines of immobilized antibodies.
Result Indicators
- Proper test performance is indicated by the formation of a blue line at the control side (C) of the Reaction Window.
- A positive result is indicated by the formation of a blue line at the test side (T) of the Reaction Window.
- If the visible line is located on the side of the cassette labeled “T,” or no lines are visible in the Reaction Window, then the test did not run properly, the results are invalid and the sample should be tested again using a fresh cassette.
Materials provided
• Membrane Devices – 25 pouches, each containing 1 device
• Diluent (14 mL) – Buffered protein solution with graduated dropper assembly (contains 0.05% ProClin® 300)
Signal Word: Warning
H317: May cause an allergic skin reaction
P261, P272, P280, P302, P352, P333, P313, P321, P362, P364, P501
• Wash Buffer (12 mL) – A buffered solution with graduated dropper assembly (contains 0.05% ProClin® 300)
Signal Word: Warning
H317: May cause an allergic skin reaction
P261, P272, P280, P302, P352, P333, P313, P321, P362, P364, P501
• Substrate (3.5 mL) – Solution containing tetramethylbenzidine
• Conjugate (2.0 mL) – Mouse monoclonal antibody specific for glutamate dehydrogenase coupled to horseradish peroxidase in a buffered protein solution (contains 0.05% ProClin® 300)
Signal Word: Warning
H317: May cause an allergic skin reaction
P261, P272, P280, P302, P352, P333, P313, P321, P362, P363, P364, P501
• Positive Control (1 mL) – Antigen in a buffered protein solution
• Disposable plastic transfer pipettes, 50 (graduated at 25 µL, 400 µL and 500 µL)
| Product Name | Suggested CPT Code | Analyte(s) Detected | Format | Time to Result | Fecal Sample Type |
| C. DIFF QUIK CHEK | 87449 | GDH | Rapid EIA | < 30 min | Fresh fecal sample (unpreserved) |
FEATURES & BENEFITS
- Easy to interpret
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Highly sensitive and specific
- Results in approximately 30 minutes
PRODUCT DETAILS
You may place order by giving us a call
| Catalog Number | Description | Units of Measurements |
| TL30390 | C. DIFF QUIK CHEK | 25 Tests/Kit |
DOCUMENTATION AND VIDEOS
Brochure, package inserts and additional literature provided below:
C. DIFF QUIK CHEK Poster Presentations
Glutamate dehydrogenase (GDH) is Highly Conserved Among Clostridium difficile Ribotypes
American Society of Microbiology meeting, May 2011, New Orleans, LA
Prevalence of Clostridium difficile in Fecal Samples from Inpatient and Outpatient Populations
American Society of Microbiology meeting, May 2011, New Orleans, LA
Glutamate Dehydrogenase is Highly Conserved Among Clostridium difficile Ribotypes
J. Clin. Microbiol. April 2012 vol. 50 no. 4 1425-1426
Detection of Clostridium difficile in fecal specimens using a new rapid antigen test, the C. DIFF QUIK CHEK®
American Society for Microbiology meeting, May 2006, Orlando, FL
Multicenter Evaluation of a Rapid Test for Detection of Clostridium difficile in Fecal Specimens
American Society for Microbiology meeting, May 2006, Orlando, FL
Two Rapid Tests for Detection of Clostridium difficile in Fecal Specimens
American Society for Microbiology meeting, June 2005, Atlanta, GA
C. difficile Diagnostics
Clostridium difficile is the major cause of nosocomial diarrhea and pseudomembranous colitis
C. difficile infection usually occurrs when the normal microflora of the intestinal tract is altered or killed by antibiotics. Once C. difficile growth begins, toxins A and B are produced, causing diarrhea and colitis.
Toxigenic strains of C. difficile carry the genes encoding the toxins. The disease results from the toxins that the organism produces. C. difficile produces two main toxins, toxin A and toxin B. Toxin A is a weakly cytotoxic enterotoxin. C. difficile also produces toxin B, which is a cytotoxin. Toxin B can be detected by the tissue culture assay. Toxigenic C. difficile strains produce both toxins or only toxin B.
The disease can be treated with antibiotics, but relapses are common.
