The Accutest Standard Wound RT-PCR Panel provides a comprehensive, multiplexed real-time PCR solution for the detection of bacterial and fungal pathogens associated with wound and soft-tissue infections. Each panel includes optimized primers and probes for 24 clinically relevant targets, covering a broad range of aerobic, anaerobic, and fungal organisms commonly linked to wound infections. An internal control is incorporated in every reaction to validate nucleic acid extraction, amplification, and detection, ensuring consistent assay reliability.

The panel is available in two formats: pre-spotted 384-well plates for high-throughput testing and tubes containing reagents for 100 reactions. This dual configuration provides flexibility for laboratories to select the format best suited to their workflow and throughput requirements.

By consolidating multiple pathogen detections into a single multiplex reaction, the Accutest Wound RT-PCR Panel reduces turnaround time and manual labor compared to culture-based methods, while conserving reagents and improving efficiency. The pre-spotted plate design eliminates manual primer and probe preparation, minimizing contamination risk and pipetting variability. All products are shipped on dry ice and stored at –15 °C to –25 °C for long-term stability and reliable performance.

Sample input may include direct specimen or purified nucleic acid, with reactions prepared in a 10 μL final volume. Both standard and fast cycling protocols are supported, allowing laboratories to balance sensitivity and runtime according to workflow needs.

The RT-PCR panel is intended for research use only and is not validated for diagnostic procedures. With broad pathogen coverage and a streamlined workflow, it offers a reliable and scalable platform for molecular wound microbiology research.

The Accutest Standard Wound RT-PCR Panel delivers multiplex detection of key bacterial and fungal pathogens involved in wound infections. Designed to detect 24 clinically relevant organisms, the panel provides broad coverage of the most common wound pathogens. Available in 384-well pre-spotted plates and tubes (100 reactions), it combines high throughput, operational flexibility, and consistent performance.

Broad-Range 4X MasterMix is a one-step RT-PCR solution optimized for multiplex applications, providing fast, reliable performance and compatibility with all Accutest RT-PCR panels.

Composition and Features

• Integrated enzymes: Hot-start reverse transcriptase and hot-start DNA polymerase for efficient cDNA synthesis and amplification.
• Balanced reaction mix: Includes optimized dNTPs, MgCl₂, proprietary enhancers, and stabilizers to maximize performance under multiplex conditions.
• One-step RT-PCR: Built-in reverse transcription reduces run time and simplifies detection of single-stranded RNA templates.

Advantages

• High performance across multiplex RT-PCR panels
• Reliable amplification from complex sample matrices
• Reduced assay time with integrated reverse transcription
• Universal compatibility with Accutest RT-PCR panel designs
• Flexible use across diverse workflows