The Accutest Standard GI RT-PCR Panel provides a comprehensive, multiplexed real-time PCR solution for the detection of gastrointestinal pathogens associated with acute diarrheal illness, foodborne transmission, and antibiotic-associated colitis. Each panel includes optimized primers and probes for 17 clinically relevant targets across bacterial, viral, and parasitic organisms commonly linked to gastrointestinal disease.

Acute gastroenteritis (“stomach bug”) can cause sudden vomiting, watery or bloody diarrhea, abdominal cramping, fever, and dehydration. The U.S. Centers for Disease Control and Prevention (CDC) identifies norovirus as the leading cause of vomiting and diarrhea in the United States and the leading cause of foodborne illness. :contentReference[oaicite:0]{index=0} Common foodborne and enteric pathogens such as Salmonella, Campylobacter, Shigella, diarrheagenic E. coli, and intestinal parasites can spread through contaminated food or water, unwashed hands, or close contact with someone who is sick, and can lead to severe dehydration or bloody diarrhea in high-risk patients. :contentReference[oaicite:1]{index=1}

The Accutest GI RT-PCR Panel is designed to support research into a range of GI syndromes — including acute infectious diarrhea, traveler’s diarrhea, foodborne outbreaks, and Clostridium difficile-associated colitis — by consolidating broad pathogen coverage into a single multiplex assay.

An internal control is incorporated in every reaction to confirm nucleic acid extraction, amplification, and detection, supporting consistency and reproducibility across runs.

The panel is available in two formats: pre-spotted 384-well plates for high-throughput testing and tubes containing sufficient reagents for 100 reactions. This dual offering provides flexibility for laboratories to select the configuration best suited to their workflow and throughput requirements.

All products are shipped on dry ice and stored at –15 °C to –25 °C to maintain stability and lot-to-lot performance. Sample input may include direct specimen or purified nucleic acids, with reactions prepared in a 10 μL final volume. Both standard and fast cycling protocols are supported, allowing labs to balance sensitivity and turnaround time according to research needs.

The RT-PCR panel is intended for research use only and is not validated for diagnostic procedures.

The Accutest Standard GI RT-PCR Panel delivers a multiplex real-time PCR workflow for gastrointestinal infection research. The panel targets 17 organisms commonly implicated in community outbreaks, foodborne transmission, daycare and long-term care facility spread, travel-related diarrhea, and antibiotic-associated colitis.

According to the Centers for Disease Control and Prevention (CDC), foodborne pathogens such as Salmonella, Campylobacter, Shigella, Escherichia coli, and norovirus are among the leading causes of gastrointestinal illness and outbreaks in the United States.

By detecting bacterial, viral, and parasitic causes of gastroenteritis in a single assay, the panel helps streamline studies of outbreak sources, co-infections, and severity drivers such as shiga-like toxin–producing E. coli, which is associated with bloody diarrhea and potential post-infectious complications.

Available in 384-well pre-spotted plates and tubes (100 reactions), the panel combines high throughput and operational flexibility, enabling accurate and efficient molecular investigations in research laboratories.

Broad-Range 4X MasterMix is a versatile one-step RT-PCR solution optimized for multiplex applications, offering fast, reliable performance and seamless compatibility with all Accutest RT-PCR panels.

Composition and Features

• Integrated enzymes: Hot-start reverse transcriptase and hot-start DNA polymerase for efficient cDNA synthesis and amplification.
• Balanced reaction mix: Includes optimized dNTPs, MgCl₂, proprietary enhancers, and stabilizers to maximize performance under multiplex conditions.
• One-step RT-PCR: Built-in reverse transcription reduces run time and supports simultaneous detection of RNA and DNA targets (including common GI viruses).

Advantages

• High performance across multiplex RT-PCR panels
• Reliable amplification from complex GI sample matrices
• Reduced assay time with integrated reverse transcription
• Universal compatibility with Accutest RT-PCR panel designs
• Flexible use across diverse workflows